Structure of arthropod hemocyanin

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Structure of arthropod hemocyanin

Hemocyanins are large multi-subunit copper proteins that transport oxygen in many arthropods and mollusts. The amino acid sequence of subunit a of Panulirus interruptus hemocyanin (657 residues) has been completed and fitted to the electron-density map (3.2 A resolution). Comparison of amino acid sequence data for several different hemocyanin subunits of arthropod species indicated that the gen...

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Centipedal hemocyanin: its structure and its implications for arthropod phylogeny.

The oxygen carrier hemocyanin occurs in the blood of Scutigera coleoptrata, a uniramous arthropod, as well as the crustaceans and chelicerates. The native polymer appears to be composed of substructures having the same size and electron-dense image as those of other arthropod hemocyanins but assembled into a unique multiple and arranged in a unique configuration. The simplest explanation of the...

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Molecular basis of the Bohr effect in arthropod hemocyanin.

Flash photolysis and K-edge x-ray absorption spectroscopy (XAS) were used to investigate the functional and structural effects of pH on the oxygen affinity of three homologous arthropod hemocyanins (Hcs). Flash photolysis measurements showed that the well-characterized pH dependence of oxygen affinity (Bohr effect) is attributable to changes in the oxygen binding rate constant, k(on), rather th...

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Molecular cloning of insect pro-phenol oxidase: a copper-containing protein homologous to arthropod hemocyanin.

Pro-phenol oxidase [pro-PO; zymogen of phenol oxidase (monophenol, L-dopa:oxygen oxidoreductase, EC 1.14.18.1)] is present in the hemolymph plasma of the silkworm Bombyx mori. Pro-PO is a heterodimeric protein synthesized by hemocytes. A specific serine proteinase activates both subunits through a limited proteolysis. The amino acid sequences of both subunits were deduced from their respective ...

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Catalytic oxygenation of phenols by arthropod hemocyanin, an oxygen carrier protein, from Portunus trituberculatus.

The hexamer (Pt-6Hc) of swimming crab Portunus trituberculatus hemocyanin (Pt-Hc) and one of its monomeric subunits (Pt-1Hc) have been purified and converted to an efficient phenol monooxygenase (phenolase) by treatment with urea. To explore the intrinsic chemical reactivity of the dicopper center of Pt-Hc, the spectroscopic features and phenol monooxygenase (phenolase) activity of the isolated...

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1986

ISSN: 0014-5793

DOI: 10.1016/0014-5793(86)81402-8